ELISA kit for the quantitative determination of aflatoxin M1 in milk, powder milk and cheese.

Overall process times were geared to requirements as defined by the USDA GIPSA test performance specifications.

Method: Competitive enzyme immunoassay

Detection limit: < 0,01 ppb
Powder milk (< 0,01 ppb) - Cheese (<0,25 ppb)

Quantification limit: 0,05 - 0,11 ppb

Assay time: 140 min

Detection: 450 nm

Recovery rate:
Milk (78%) - Powder milk (nd) - Cheeses (83%) - Yogurt (89%)

Standards: 0; 0,01; 0,05; 0,1; 0,5; 1 [ppb]

Ref. Range: 0 – 1 ppb

Cross reaction:
- Aflatoxin B1 10%
- Aflatoxin G1 5%
- Aflatoxin B2 3,5%
- Aflatoxin G2 2,1%
- Sterigmatocystin 0,02%

Storage temperature: 2 - 8ºC

Format: Microplate - 96 wells

- Quantitative results based on superior 4PL curve fitting for calibration
- Microsoft Excel-based calculation & evaluation tool available
- Reagents (excl. wash buffer) ready-to-use
- 96 well format with breakable microwell strips
- Simple extraction procedures
- Incubation at room temperature
- High accuracy and reproducibility
- High stability; minimum shelf life 12 months

Milk, powder milk and cheese